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a DNA-PK + PAXX + Pol λ dimeric map with extra density for Pol λ highlighted in a dashed purple circle, with two orientations of processed half dimer presented below. <t>DNA-PKcs</t> in gray, Ku70 in orange, Ku80 in green, DNA in yellow, PAXX in cyan, and Pol λ in purple. b DNA-PK + PAXX + Pol λ + LX4 with extra density for Pol λ highlighted in a dashed purple circle, with two orientations of processed half dimer presented below. DNA-PKcs in gray, Ku70 in orange, Ku80 in green, DNA in yellow, LigIV in red, XRCC4 in dark blue, PAXX in cyan, and Pol λ in purple. Inset, zoom in of the Pol λ extra density. Source data are provided as a file.
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a DNA-PK + PAXX + Pol λ dimeric map with extra density for Pol λ highlighted in a dashed purple circle, with two orientations of processed half dimer presented below. <t>DNA-PKcs</t> in gray, Ku70 in orange, Ku80 in green, DNA in yellow, PAXX in cyan, and Pol λ in purple. b DNA-PK + PAXX + Pol λ + LX4 with extra density for Pol λ highlighted in a dashed purple circle, with two orientations of processed half dimer presented below. DNA-PKcs in gray, Ku70 in orange, Ku80 in green, DNA in yellow, LigIV in red, XRCC4 in dark blue, PAXX in cyan, and Pol λ in purple. Inset, zoom in of the Pol λ extra density. Source data are provided as a file.
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a DNA-PK + PAXX + Pol λ dimeric map with extra density for Pol λ highlighted in a dashed purple circle, with two orientations of processed half dimer presented below. <t>DNA-PKcs</t> in gray, Ku70 in orange, Ku80 in green, DNA in yellow, PAXX in cyan, and Pol λ in purple. b DNA-PK + PAXX + Pol λ + LX4 with extra density for Pol λ highlighted in a dashed purple circle, with two orientations of processed half dimer presented below. DNA-PKcs in gray, Ku70 in orange, Ku80 in green, DNA in yellow, LigIV in red, XRCC4 in dark blue, PAXX in cyan, and Pol λ in purple. Inset, zoom in of the Pol λ extra density. Source data are provided as a file.
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a DNA-PK + PAXX + Pol λ dimeric map with extra density for Pol λ highlighted in a dashed purple circle, with two orientations of processed half dimer presented below. <t>DNA-PKcs</t> in gray, Ku70 in orange, Ku80 in green, DNA in yellow, PAXX in cyan, and Pol λ in purple. b DNA-PK + PAXX + Pol λ + LX4 with extra density for Pol λ highlighted in a dashed purple circle, with two orientations of processed half dimer presented below. DNA-PKcs in gray, Ku70 in orange, Ku80 in green, DNA in yellow, LigIV in red, XRCC4 in dark blue, PAXX in cyan, and Pol λ in purple. Inset, zoom in of the Pol λ extra density. Source data are provided as a file.
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Image Search Results


a DNA-PK + PAXX + Pol λ dimeric map with extra density for Pol λ highlighted in a dashed purple circle, with two orientations of processed half dimer presented below. DNA-PKcs in gray, Ku70 in orange, Ku80 in green, DNA in yellow, PAXX in cyan, and Pol λ in purple. b DNA-PK + PAXX + Pol λ + LX4 with extra density for Pol λ highlighted in a dashed purple circle, with two orientations of processed half dimer presented below. DNA-PKcs in gray, Ku70 in orange, Ku80 in green, DNA in yellow, LigIV in red, XRCC4 in dark blue, PAXX in cyan, and Pol λ in purple. Inset, zoom in of the Pol λ extra density. Source data are provided as a file.

Journal: Nature Communications

Article Title: Structural and functional insights into the interaction between Ku70/80 and Pol X family polymerases in NHEJ

doi: 10.1038/s41467-025-59133-2

Figure Lengend Snippet: a DNA-PK + PAXX + Pol λ dimeric map with extra density for Pol λ highlighted in a dashed purple circle, with two orientations of processed half dimer presented below. DNA-PKcs in gray, Ku70 in orange, Ku80 in green, DNA in yellow, PAXX in cyan, and Pol λ in purple. b DNA-PK + PAXX + Pol λ + LX4 with extra density for Pol λ highlighted in a dashed purple circle, with two orientations of processed half dimer presented below. DNA-PKcs in gray, Ku70 in orange, Ku80 in green, DNA in yellow, LigIV in red, XRCC4 in dark blue, PAXX in cyan, and Pol λ in purple. Inset, zoom in of the Pol λ extra density. Source data are provided as a file.

Article Snippet: Primary antibodies used: mouse monoclonal antibodies anti-DNA-PKcs (clone 18.2; Thermo Fisher Scientific, MA5-13238, lot: Y14047369), anti-Ku80 (clone 111; Thermo Fisher Scientific, MA5-12933, lot: WB31922872), anti-Ku70 (clone N3H10; Thermo Fisher Scientific, MA5-13110, lot: WE27669780), anti-Pol lambda (clone E11; Santa Cruz, sc-373844, lot: B0818), anti-beta-Actin (clone C4; Santa Cruz, sc-47778, lot: A2023); rabbit monoclonal antibodies anti-LigIV (Abclonal, A11432, lot: 4000000595) and anti-Pol mu (Abcam, EPR10470 (B), lot: GR117969-3); rabbit polyclonal antibodies anti-XLF (Abclonal, A19957, lot: 00040701118) and anti-PAXX (Novus, NBP1-94172, lot: C118006).

Techniques: